The National Institute of Information and Communications Technology (NICT), the Japan Science and Technology Agency (JST), Toin University of Yokohama, and Chiba University have succeeded in developing a color-multiplexed holography system by which 3-D information of objects illuminated by a white-light lamp and self-luminous specimens are recorded as a single multicolor hologram by a specially designed and developed monochrome image sensor.
They achieved single-shot color-multiplexed fluorescence holographic microscopy by exploiting digital holography and computational coherent superposition (CCS). The developed microscope acquires the color 3-D information of self-luminous objects with a single-shot exposure and no color filter array. The system does not need a laser light source, and has the capability to conduct multicolor 3-D motion-picture sensing of biological samples and moving objects at video rate. The researchers hope to develop multi-color 3-D microscopy of extremely weak light such as autofluorescence and nonlinear light.
This achievement was published in Applied Physics Letters as an open-access paper on July 22, 2020.
Simultaneous color 3-D sensing of multiple self-luminous objects was demonstrated by recording a single color-multiplexed hologram of fluorescence light. Multicolor 3-D imaging with a white-light lamp was also demonstrated with a single-hologram recording. The researchers exploited CCS, a holographic multiplexing technique, to record a color-multiplexed hologram on a monochrome image sensor without any color filter. Only a single-shot exposure is required to conduct color fluorescence holographic 3-D sensing using the system. The number of exposures required to acquire the information is 250 times less than of that required for a conventional color-multiplexed fluorescence holographic microscope.
The researchers next want to increase the recording speed and develop multicolor holographic 3-D motion-picture image sensing for spatially incoherent light.
Tatsuki Tahara et al, Single-shot wavelength-multiplexed digital holography for 3D fluorescent microscopy and other imaging modalities, Applied Physics Letters (2020). DOI: 10.1063/5.0011075
National Institute of Information and Communications Technology (NICT)
Instantaneous color holography system for sensing fluorescence and white light achieved (2020, July 22)
retrieved 22 July 2020
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